Research on genes expression, SNP genotyping or detection of viral or bacterial DNA can be done without any problem.

 Fast, efficient and reproducible method of nucleic acids analysis in real time (Real-time PCR) using the latest generation devices such as QuantStudio 6, Viia7, 7900 Real-Time PCR System from Applied Biosystems. Access to removable 96 and 384-well blocks gives the ability to analyze hundreds of samples and several features simultaneously (e.g. multiplex gene expression analysis). It allows to save time and reduce the quantity of template (sample). Additionally, QuantStudio 6 is capable to perform HRM (High Resolution Melting) analysis.

Real-time PCR is very fast and efficient method of nucleic acids analysis, allowing to detect RNA and DNA, as well as accurately measure concentration. PCR reaction is prepared to amplify specific, single-strand fragment of DNA/cDNA template and multiply copy numbers. The detection of signal from amplicons is possible due to addition of fluorochromes (fluorescent days). Advanced optical systems (halogen lamp, bright white LED or laser and spectrograph and CCD camera or OptiFlex™ System - depending on the equipment) induce fluorescence and allow detection of signals released by different dyes, among others, FAM ™, SYBR® Green, VIC®, JOE ™, NED ™, TAMRA ™, Cy3 ™, ROX ™, Texas Red®, Cy5 ™. High sensitivity allows to detect even small differences in gene expression between samples or find a single mutations in a gene. Reliable and reproducible results can be obtained during a single 1-2 hours-long analysis using specific primers, probes and reagent kits containing, e.g. Taq DNA polymerase. QuantStudio 6 Flex Real-Time PCR System can alfo perform HRM (High Resolution Melting) analysis.

More info on manufacturer’s website: QuantStudio 6

Applied Biosystems Real – Time 7900 and Viia 7 are located at the DNA Lab (Room A22b, Bydgoska str. 7).
QuantStudio 6 is located at the Department of Immunology and Food Microbiology (Room 121, Tuwima str. 10).