Advanced in vitro experiments cannot be performed without cell manipulation, e.g., gene introduction or cell membranes fusion.

Gene Pulser Xcell can be used in electroporation and electrofusion experiments. The electromagnetic field generated by this device can change the permeability of the membrane allowing vectors, nucleic acids and other molecules to enter into living cells; pro- and eukaryotic. Fortunate it is a modular electroporation system for transfecting every cell type. The system includes a main unit, a ShockPod™ cuvette chamber, and two accessory modules: the capacitance extender (CE module) and the pulse controller (PC module). The CE module is used for low voltage (500 V) condenser, allowing the electroporation of mammalian cells and protoplasts of plant cells. The PC module produces a much higher voltage (3000 V) necessary to operate the fungi and bacterial cells. It has a large number of parameters that can be easily adjusted (voltage, number of pulses, duration or time interval between pulses). Gene Pulser Xcell™ Electroporation System can be widely used in various types of in vitro experiments when quick, efficient and reproducible modifications of cells is needed.

More info on manufacturer’s website: read

Gene Pulser Xcell™ Electroporation System is located at the Bacteria and Fungi Lab (Room A21).